The columns of choice for ionpair or ionsuppression chromatography, these polymerbased, reversedphase columns provide excellent resolution and peak shape. Learn about our latest advancements in ic by visiting our innovations in ion chromatography resource center. The modular nature of the thermo scientific dionex ultimate 3000 platform does allow modifications to this layout. The waters chromatography columns and supplies catalog contains many of todays leading separation technology products that feature the latest innovations for sample preparation, column technologies, and biomolecule characterization solutions. Ion chromatography columns thermo fisher scientific us. Thermo scientific dionex aers 500 carbonate suppressors. There are four questions to consider when choosing an hplc. Designed for improved chromatography, thermo scientific hypersil gold columns are the culmination of over 35 years of experience in the product. Acclaim bonded silicabased columns for hplc dionex. Access the latest version of our chromatography consumables catalog in pdf and online formats. Reversedphase analysis of a monoclonal antibody on an.
Underivatized carbohydrates are separated at high ph, in an approach that is unique to dionex, simple to reproduce, and. Biosystems engineering hplc standard operating procedure. C18 hplc columns are the most preferred as they offer an excellent range of hydrophobic separation power along with high surface area coverage. Thermo scientific dionex ion chromatography ic systems have led the industry for over 30 years, with instruments and solutions that represent stateoftheart technological advancements and. The technology made by dionex includes the rapid separation lc rslc and polymeric hplc columns, a type of monolithic hplc column. Most c18 hplc columns undergo phase collapse in 100% aqueous mobile phases with concomitant loss of retention. Column 1 column 2 waste product waste hplc 23129 in the final step of the process, the columns continue to be operated in parallel mode as shown in figure 7.
Achieve highresolution separation of proteins and peptides under nondenaturing conditions with thermo scientific propac hic10 lc columns. Dionex has played a pioneering role in the field of nano hplc, a technique that generally involves the application of columns with an internal diameter of 75 m and low flow rates of around 300 nlmin. Thermo scientific dionex chromeleon chromatography. Hplc columns for determining atp and adp chromatography. The dionex acclaim 120 columns use ultrapure silica with a stable bonding procedure to deliver a single line of columns that are lc ms compatible. Packing is a neutral, macroporous, high surface area, ethylvinylbenzene polymer cross linked with 55% divinylbenzene, making ionpac ns1 columns resistant to solvents, acids and bases. Controller specifications link to buck operators manuals pdf 219 pgs buckchrom controller gradient range 0100% increments 0. Typically, agilent hiplex columns use isocratic conditions with water as the mobile phase and temperature as the main variable for resolution control.
Ultimate 3000 scope the scope of this document is to present an overview of the high performance liquid chromatography hplc system ultimate 3000 and to provide the reader with guidelines for standard operations, routine maintenance and simple trouble shooting. View and download thermo scientific dionex ultimate 3000 series operating instructions manual online. Agilent infinitylab solutions for high and ultrahighperformance liquid chromatography include instruments, columns, and supplies to help your lab achieve highest operational efficiency. The strategy of surfactant analysis by hplc xiadong liu, mark tracy, and christopher pohl dionex corporation, sunnyvale, ca, usa introduction surfactants are widely used in the consumer. Unlike the inorganic silica columns, the polymer monoliths are made. View the selectivity profiles of each phase, pages 1222, to see how each column can provide you with a truly different selectivity. Ankur choudhary print question forum 8 comments columns are the main component in hplc because the column is responsible for the separation of the sample components. Ultimate 3000 nano and cap system installation and application manual.
Yes, depending on autosampler capabilities available with as autosampler dilution factor, as autosampler. Rpamide hplc columns, polar embedded phase 67 phenyl hplc columns, alternate selectivity 29 pentafluorophenyl hplc columns, unique selectivity 50 diol hplc columns 8 cyano hplc columns, for basic and polar analytes 15 amino hplc columns 20 silica hplc columns, high loading capacity 33 hilic or anp aqueous normal. Featuring exp lc guard columns and connectors from optimize technologies, restek offers a full complement of hardware to extend the life of your restek lc columns. This causes a problem when trying to analyze very polar compounds. C4 and c5 hplc columns are commonly used to separate large macromolecules such as proteins. For a more detailed discussion of the subject, the reader is referred to textbooks,14 magazine columns,5 training software,6 manufacturers operating and service manuals, and myriad. Sequentialsimultaneous injection dionex asdv, as, ashv, or third party autosamplers automated dilution. Thermo scientific dionex ionpac as11 and as11hc anionexchange column. Thermo scientific dionex ultimate 3000 series operating.
For a more detailed discussion of the subject, the reader is referred to textbooks,14 magazine columns,5 training software,6 manufacturers operating and service manuals, and myriad web resources. Acclaim mixedmode hilic1 column crawford scientific. Introduction hplc lthough hplc method has been improved by. Standard operating procedure thermo scientific ultimate 3000. Column 1 undergoes regeneration while the highpurity product is automatically collected from column 2. The dionex carbopac pa10 column resolves mixtures of sugar alcohols. Reversedphase analysis of a monoclonal antibody on an accucore 150c4 hplc column ken cook, joanna freeke, valeria barattini, thermo fisher scientific, runcorn, cheshire, uk introduction. Spring savings coming soon selected hilic, ic, sugar, sec series register to win complete winner will be announced next week. Hypersil gold hplc columns phase overview thermo scientific. The dionex autotrace 280 instrument automates the cartridge or disk conditioning, sample loading. I found literatures which indicate the columns that the authors used e.
Resteks line of hplc and uhplc columns includes the raptor, force, roc, ultra, pinnacle db, and viva product families available with the convenience of uslc column phases. Ultimate 3000 rslcnano standard application manual. New chromatography columns and accessories for 2016. Shodex hplc columns technical support for the americas. From routine analysis to cuttingedge research, the infinitylab lc series offers the broadest portfolio of hplc and uhplc solutions for any application and. Bob morrison stlccfv, instrumentation specialist original april 09, latest revision june 2014 buckchrom blc20g plus software system educationoverview of hplc. With the dionex aminotrap column inline, the dionex carbopac pa10 column offers lowered detection limits, elimination of interference from oxygen, and faster run times. I get reproducible separations from column lottolot at 52.
You can now streamline your chromatography and ms quantitation workflows in one application, making your lab more efficient. Analysis of carbohydrates, alcohols, and organic acids. You will be logged off in seconds due to inactivity. Thermo scientific chromatography columns and consumables. Online hplc as a process analytical technology pat for. The dionex ionpac as11 and as11hc anionexchange columns are designed for the separation of organic acids and inorganic anions in various matrices.
To condition the column prior to separation, choose the appropriate starting mobile phase composition by adjusting the percentages of each appropriate eluent scale note that you. Dionex has established high standards to ensure high quality from their products and services. Simpler matices can quickly be profiled using the as11 while the as11hc is best for more complex, higher ionic strength matrices. Dionex ultimate 3000 series laboratory equipment pdf manual. During operation the front cover of the column compartment should be closed, especially if temperature control is applied nb.
Dionex acclaim columns are silicabased columns designed for high efficiency separations and manufactured using ultrahigh purity silica. Equilibration of hplc columns mourne training services. The ultimate 3000 standard systems with their perfect interplay. The acclaim mixedmode hilic1 column is a silicabased packing material that incorporates both reversedphase rp. Care, maintenance, and troubleshooting of hplc columns. The enhanced online version features a robust applications search tool, access to method development guides, application notebooks, and much more. Part old part number in this manual updated part number to use for new orders prod,col,ip,atc3,4x35mm 059661 079932 prod,col,ip,utaclp2,4x35mm 072779 079917. Superior column protection for uplc, uhplc, and hplc columns. Ultimate 3000 standard operational procedure jagger page 9 of 21 edition 01 figure 9. Note that slight variations may be obtained on tow different hplc systems.
Ultimate 3000 proteomics mdlc system installation and application manual. Developed with multifunctional ligand attachments, these silicabased columns provide exceptional hydrolytic stability under the highly aqueous conditions used in hic. The selection of a highly reproducible hplc column is essential if this goal is to be attained. Dionex acclaim e2 column issue scaling from hplc to uhplc. Eluents can be delivered by reagentfree ion chromatography. Chromatography columns and consumables catalog 20162017. Waters uplc, uhplc, and hplc column selection and mobile.
Dionex ionpac columns are ionpac polymeric columns for use with hydroxide, carbonate, and methanesulfonic acid eluents. Variety of selectivities and solid supports for rphplc methods. Ultimate 3000 rslcnano standard applications 3 1 introduction this document describes the standard setup, recommended experimental conditions and testing procedures to run standard applications on the thermo scientific dionex ultimate 3000 rslcnano system. Operating the column compartment the column compartment is the module where the column is connected. The thermo scientific dionex autotrace 280 solidphase extraction spe instrument is a highthroughput workstation dedicated specifically for automating spe. Allows method scaling from microbore through preparative column range.
Thermo scientific columns meet the stringent requirements of modern hplc and lcms. The ultimate guide to hplcuhplc phenomenex uhplc, hplc. Thermo scientific dionex products have a reputation for leading innovation with excellence in ion chromatography ic, liquid chromatography hplc and uhplc, automated sample preparation solutions, and chromatography data system software. Online ordering register now and save browse polymerbased hilic columns shodex polymerbased columns, manufactured by showa denko, contain rugged and optionally functionalized sdvb, polyhydroxymethacrylate or pva based solid supports that are longerlasting and. Usp listing of hplc columns l packingbrand name brand name l37 l38 l39 l40 l41 l43 l44 l51 l48 l49 l52 l53 l54 l55 l56 l57 l58 l59 l60 l61 packing having the capacity to separate proteins. Fittings on all hplc and uhplc columns have 1032 threads. Dionex ultimate 3000 series laboratory equipment pdf manual download. Detailed instrument description can be found in dionex. This technical bulletin describes how discovery bio wide pore c18 columns do not undergo phase collapse, and can be used in 100% aqueous conditions. Syncronis c18 hplc columns thermo fisher scientific. Here, this is defined as a system capable of operating up to 8,700 psi 600 bar 3 uplc technology defined as the use of sub2 m particle columns in. Equilibration of hplc columns friday, 1 may 2009 peak solutions a resource for chromatographers how long should i leave my hplc column to equilibrat hplc analysts, particularly those relatively new.
Thermo scientific dnapac pa100 oligonucleotide columns. Dec, 2007 this is a topic i wish uwe was still around for i have been running a column from dionex for years the acclaim e2 250x4. Manual decrimper removes 8mm aluminum crimp seals without vial damage. A sugar alcohols and other carbohydrates found in foods.
Dionex ultimate 3000 rslcnano standard applications. Hplc column switching switcher valve dionex, column. Save thousands of dollars over other valve systems. Though the chart below depicts several similar bonded ligand types, no two columns are the same. Ics1600 ion chromatography system dionex pdf catalogs. The chart lists the columns in descending order of hydrophobic capacity h. Ultimate 3000 scope the scope of this document is to present an overview of the high performance liquid chromatography hplc system ultimate 3000 and to provide the reader with. Aminex hplc columns, which are packed with a polymerbased matrix, are the research and industry standard for the analysis of carbohydrates, alcohols, and organic acids in food and beverage. Calculates conditions for transfer of an isocratic or gradient method from one hplc column to another. Different types of hplc columns are used in analysis of different pharmaceutical compounds according to their nature and column separation capacity. C8 hplc columns are used when less retention compared to a c18 is needed.
Dionex ultimate 3000 standard systems thermo fisher scientific. Size exclusion chromatography sec is a chromatographic method which separates analytes solely based. Rpamide hplc columns, polar embedded phase 67 phenyl hplc columns, alternate selectivity 29 pentafluorophenyl hplc columns, unique selectivity 50 diol hplc columns 8 cyano hplc. Among the many c18 columns on the market, retention behavior of analytes differs significantly due to variation in both the raw silica and the ligand bonding chemistry. Dionex ultimate 3000 rslcnano standard applications manual. Product manual for dionex ionpac as20 and ag20 columns 06504405 for new orders of the following parts discussed in this manual, please use the updated part numbers listed below. The acclaim 120 line of hplc columns is a new line of c18 and c8 silicabased, reversedphase columns manufactured by dionex using exhaustive bonding and endcapping techniques. Figure 4 shows the separation of a mixture of pesticides in less than 4 min. Feature strong anionexchange resin for highresolution analysis and purification of synthetic and modified oligonucleotides with thermo scientific dionex dnapac pa100 oligonucleotide. Dionex, owned by thermo scientific, is a leading global provider of bioanalytical technologies and equipment for the pharmaceutical, bioscience, medical science, and food industries. Thermo scientific dionex carbopac pa1 and pa10 columns for. Consult thermo scientifics entire acclaim bonded silicabased columns for hplc catalogue on medicalexpo.1553 717 342 11 914 571 1349 1117 645 1451 1088 93 707 551 1210 254 854 14 1535 1476 1531 350 300 119 51 208 1327 684 212 1255 781 337 1054